Human ENG (CD105) knockout HeLa cell lysate
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ENG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 19 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
ENG, CD105, Endoglin, END
- WB
Lab
Western blot - Human ENG (CD105) knockout HeLa cell lysate (AB256906)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : ENG knockout HeLa cell lysate (20 μg)
Lane 3 : HUVEC cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab170943 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CD105 antibody [EPR10145-10] (<a href='/en-us/products/primary-antibodies/cd105-antibody-epr10145-10-ab170943'>ab170943</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ENG knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ENG (CD105) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-eng-cd105-knockout-hela-cell-line-ab265178'>ab265178</a>)
Lane 3:
HUVEC cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70-120 kDa
false
- WB
Lab
Western blot - Human ENG (CD105) knockout HeLa cell lysate (AB256906)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : ENG knockout HeLa cell lysate (20 μg)
Lane 3 : HUVEC cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab169545 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CD105 antibody [EPR10145-12] (<a href='/en-us/products/primary-antibodies/cd105-antibody-epr10145-12-ab169545'>ab169545</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ENG knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ENG (CD105) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-eng-cd105-knockout-hela-cell-line-ab265178'>ab265178</a>)
Lane 3:
HUVEC cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70-120 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)
Allele-2 : 11 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)
Allele-1 : 19 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)
Allele-3 : 1 bp insertion in exon2
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.
Pathways
CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com