Human ENPP2 (ATX) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
ENPP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon10 and 8 bp deletion in exon10.
View Alternative Names
ATX, ATX X, Autotaxin, Autotaxin t, ENPP2_HUMAN, Ectonucleotide pyrophosphatase/phosphodiesterase 2, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, FLJ26803, LysoPLD, NPP2, PD IALPHA, PDNP2, Phosphodiesterase I alpha, Phosphodiesterase I/nucleotide pyrophosphatase 2, Plasma lysophospholipase D
- Sanger seq
Unknown
Sanger Sequencing - Human ENPP2 (ATX) knockout HeLa cell lysate (AB257424)
Allele-1 : 29 bp deletion in exon10
- Sanger seq
Unknown
Sanger Sequencing - Human ENPP2 (ATX) knockout HeLa cell lysate (AB257424)
Allele-2 : 8 bp deletion in exon10
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.
Pathways
ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com