Human EPHA2 (Eph receptor A2) knockout HCT116 cell lysate
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EPHA2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and Insertion of the selection cassette in exon5.
View Alternative Names
ARCC2, AW545284, CTPA, CTPP1, CTRCT6, EC 2.7.10.1, ECK, EPH Receptor A2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Sek-2, Soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2
- Sanger seq
Unknown
Sanger Sequencing - Human EPHA2 (Eph receptor A2) knockout HCT116 cell lysate (AB257427)
Allele-2 : Insertion of the selection cassette in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human EPHA2 (Eph receptor A2) knockout HCT116 cell lysate (AB257427)
Allele-1 : 1 bp insertion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.
Pathways
Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB273118
Anti-Eph receptor A2 antibody [EPR24711-11]
20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size
![Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (AB273118)](https://content.abcam.com/products/images/eph-receptor-a2-antibody-epr24711-11-ab273118--western-blot-img177491.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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