Human EPHA4 (Eph receptor A4/SEK) knockout HeLa cell lysate
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EPHA4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
View Alternative Names
Cek 8, EK8, EPH Receptor A4, EPH-like kinase 8, EPHA4_HUMAN, Ephrin type-A receptor 4, HEK 8, Receptor protein-tyrosine kinase HEK8, SEK 1, TYRO 1 protein tyrosine kinase, TYRO1, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK
- Sanger seq
Unknown
Sanger Sequencing - Human EPHA4 (Eph receptor A4/SEK) knockout HeLa cell lysate (AB258410)
Homozygous : 1 bp insertion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EphA4 plays a critical role in developmental processes particularly in the development of the nervous system and vascular patterning. The receptor engages in bidirectional signaling meaning that signals are transmitted not only into the receptor-expressing cells but also into the ephrin-expressing ones. EphA4 is part of complexes that can include multiple receptor and ephrin molecules which allows for the modulation of cell adhesion and migration. These processes are essential for forming tissue boundaries and axon guidance.
Pathways
EphA4 signaling is involved in two significant pathways: the Eph/ephrin signaling pathway and the cytoskeletal remodeling pathway. In the Eph/ephrin signaling pathway EphA4 interacts with various ephrin ligands leading to changes in cellular architecture and behavior. The cytoskeletal remodeling pathway involves interactions with proteins such as Rho and Ras which coordinate cytoskeletal changes necessary for cell movement and positioning. These pathways are integral to processes like axon guidance and cell migration underpinning EphA4’s role in development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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