Human ERF knockout HEK-293T cell lysate
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ERF KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.
View Alternative Names
ERF_HUMAN, ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE-2
- Sanger seq
Unknown
Sanger Sequencing - Human ERF knockout HEK-293T cell lysate (AB258861)
Allele-2 : Insertion of the selection cassette in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human ERF knockout HEK-293T cell lysate (AB258861)
Allele-1 : 1 bp insertion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ets2 repressor factor plays a role in growth regulation. It is known to negatively control the activity of other ETS transcription factors restricting their ability to activate gene expression linked with cell proliferation and differentiation. ERF is not usually part of a larger complex but can form interactions with other regulatory molecules to fine-tune cellular processes. By influencing these pathways ERF helps maintain balanced cell growth and prevent uncontrolled division.
Pathways
ERF integrates into the MAPK/ERK signaling pathway influencing cell cycle control and differentiation. This pathway also involves other proteins such as ERK2 and c-Fos which are important for transmitting signals from extracellular stimuli to the nucleus. Additionally ERF is connected to the TGF-beta signaling pathway where it aids in modulating responses to growth factors that are important in developmental processes and cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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