Human ERI3 knockout HeLa cell lysate
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ERI3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 279 bp deletion in exon1.
View Alternative Names
ERI1 exoribonuclease 3, ERI1 exoribonuclease family member 3, ERI3_HUMAN, Enhanced RNAi three prime mRNA exonuclease homolog 3, Exoribonuclease 3, FLJ22943, H_RG158K20.1, MGC2683, PINT1, PRNPIP, Prion interactor 1, Prion protein-interacting protein, WUGSC:H_RG158K20.1
- Sanger seq
Unknown
Sanger Sequencing - Human ERI3 knockout HeLa cell lysate (AB263190)
Homozygous : 279 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ERI3 decapsulates mRNA influencing mRNA stability and turnover. This function positions ERI3 as essential in mRNA decay mechanisms. ERI3 doesn't work in isolation; it forms part of a multi-protein complex involved in mRNA decay and RNA processing activities. Studies suggest that it interacts directly with other key proteins in these complexes contributing to its biological significance.
Pathways
ERI3 plays a role in RNA degradation and nonsense-mediated mRNA decay pathways. It contributes to these pathways by interacting with proteins such as DCP2 and XRN1 which are important for mRNA turnover. This involvement ensures that cells maintain their mRNA levels and quality affecting overall protein synthesis necessary for cellular function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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