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AB263192

Human ETNK1 (ethanolamine kinase) knockout HeLa cell lysate

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ETNK1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
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Sanger Sequencing - Human ETNK1 (ethanolamine kinase) knockout HeLa cell lysate (AB263192)
  • Sanger seq

Unknown

Sanger Sequencing - Human ETNK1 (ethanolamine kinase) knockout HeLa cell lysate (AB263192)

Homozygous : 1 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265219

Human ETNK1 (ethanolamine kinase) knockout HeLa cell line

Sanger Sequencing - Human ETNK1 (ethanolamine kinase) knockout HeLa cell line (AB265219)

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Primary Antibodies

AB184714

Anti-PHD3 antibody [EPR17869]

RabMAb|Recombinant

Western blot - Anti-PHD3 antibody [EPR17869] (AB184714)

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Primary Antibodies

AB236615

Anti-ethanolamine kinase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ethanolamine kinase antibody (AB236615)

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Primary Antibodies

AB113077

Anti-PHD1/prolyl hydroxylase antibody [EPR2746]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (AB113077)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ETNK1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ethanolamine kinase often referred to as ETNK1 is an enzyme known for its role in phospholipid metabolism. It catalyzes the phosphorylation of ethanolamine to produce phosphoethanolamine. This enzyme has a molecular weight of approximately 45 kDa. Ethanolamine kinase is expressed in various tissues including liver and brain indicating its importance in systemic metabolic processes.
Biological function summary

The enzyme is involved in the Kennedy pathway which is responsible for the biosynthesis of phosphatidylethanolamine an important membrane lipid. Ethanolamine kinase operates independently and is not part of any larger protein complex. It supports cellular functions including membrane formation and repair which are critical for proper cell function and signaling.

Pathways

Ethanolamine kinase plays a significant role in the phospholipid biosynthesis pathway and the related glycerophospholipid metabolism pathway. In these pathways it interacts with other enzymes like CTP:phosphoethanolamine cytidylyltransferase shaping the production and regulation of membrane phospholipids. These pathways are essential for maintaining cellular membrane integrity and fluidity.

Ethanolamine kinase has been linked to neurological disorders. Abnormalities in its function can lead to issues in brain phospholipid metabolism potentially contributing to conditions like Alzheimer's disease. Additionally alterations in ethanolamine kinase expression or activity might impact proteins involved in neurodegenerative processes further influencing disease progression.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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