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AB258865

Human FAM120A (OSSA) knockout HEK-293T cell lysate

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FAM120A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
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Sanger Sequencing - Human FAM120A (OSSA) knockout HEK-293T cell lysate (AB258865)
  • Sanger seq

Unknown

Sanger Sequencing - Human FAM120A (OSSA) knockout HEK-293T cell lysate (AB258865)

Homozygous : Insertion of the selection cassette in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

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Complementary Products

Primary Antibodies

AB156695

Anti-FAM120A/OSSA antibody

Immunoprecipitation - Anti-FAM120A/OSSA antibody (AB156695)

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Cell Lines & Lysates

AB266524

Human FAM120A (OSSA) knockout HEK-293T cell line

Sanger Sequencing - Human FAM120A (OSSA) knockout HEK-293T cell line (AB266524)

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Cell Lines & Lysates

AB258523

Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate

Western blot - Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate (AB258523)

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Cell Lines & Lysates

AB94296

SOCS5 overexpression 293T lysate (whole cell)

Western blot - SOCS5 overexpression 293T lysate (whole cell) (AB94296)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
FAM120A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM120A also known as OSSA is a protein that plays a role in cellular signaling. It has a mass of approximately 120 kDa. Researchers observe the expression of this protein in various tissues including testis ovary and brain. FAM120A functions as a scaffolding protein which means it helps organize other proteins at specific locations within cells to facilitate interactions and signaling pathways.
Biological function summary

FAM120A interacts with numerous cellular components and exhibits roles in regulating stress response. It integrates into the RNA granule complex allowing it to influence RNA stability and translation. Through its interaction with the cytoplasmic polyadenylation element-binding proteins it modulates mRNA processing offering insight into how cells manage protein production in response to environmental changes.

Pathways

FAM120A becomes integrated into signaling cascades like the PI3K/Akt pathway. It influences cell survival and growth by modulating interactions with proteins such as Src and Grb2. These interactions contribute to the regulation of key signaling activities that impact various cell functions including proliferation and apoptosis.

FAM120A has connections to cancer and cardiomyopathies. Alterations in its expression or function may affect disease pathogenesis. For instance its relationship with Src contributes to oncogenic signaling pathways influencing cancer progression. In cardiac diseases this protein interacts with pathways that involve binding partners like PI3K where its dysregulation can lead to aberrant heart function or pathology.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com