Human FAM162A knockout HEK-293T cell lysate
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FAM162A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
View Alternative Names
C3orf28, Chromosome 3 open reading frame 28, DC16, E2-induced gene 5 protein, E2IG5, F162A_HUMAN, Family with sequence similarity 162, member A1, Growth and transformation-dependent protein, HGTD P, HIF-1 alpha-responsive proapoptotic molecule, OTTHUMP00000215578, OTTHUMP00000215579, OTTHUMP00000215580, Protein FAM162A
- Sanger seq
Unknown
Sanger Sequencing - Human FAM162A knockout HEK-293T cell lysate (AB263195)
Homozygous : 2 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FAM162A is involved in promoting apoptosis through the intrinsic mitochondrial pathway. It assists in the release of cytochrome c from mitochondria which then activates caspase cascades. FAM162A does not appear to function as part of a larger protein complex; rather it operates independently to exert its pro-apoptotic effects. Its expression levels increase in response to hypoxic conditions indicating a specific role in hypoxia-induced apoptosis.
Pathways
FAM162A significantly impacts the apoptosis and hypoxia response pathways. In these pathways it interacts with proteins like cytochrome c and apoptosome components. Its action in the mitochondrial pathway suggests its role in regulating cell death in response to cellular stress making it an important component of the intrinsic apoptotic signaling pathway. FAM162A might also interplay with anti-apoptotic proteins such as Bcl-2 influencing the balance between cell survival and death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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