Human FAM83D knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
FAM83D KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
2310007D09Rik, BB104611, C20orf129, CHICA, FA83D_HUMAN, FLJ38341, Family with sequence similarity 83, member D, MGC92947, Protein FAM83D, RGD1565583, RP23-119H5.1, Spindle protein CHICA, dJ616B8.3
- Sanger seq
Unknown
Sanger Sequencing - Human FAM83D knockout HEK-293T cell lysate (AB258869)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FAM83D participates in vital cellular functions related to mitosis and cancer cell proliferation. It does not function alone but is part of the spindle assembly complex ensuring proper mitotic spindle formation and stability. These interactions highlight its importance in maintaining genomic integrity during cell division reducing the potential for errors that can lead to tumorigenesis. The involvement in this complex makes FAM83D an attractive target for studying cancer biology.
Pathways
FAM83D interacts closely with several key proteins governing cell cycle progression including the Aurora kinase pathway and the Plk1 pathway. It plays a role in phosphorylating and activating proteins necessary for mitotic entry and progression. FAM83D's interaction with Aurora B kinase is particularly critical as it contributes to proper chromosomal alignment and segregation linking it to important regulatory mechanisms in cell proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com