Human FANCD2 knockout HeLa cell lysate
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FANCD2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 13.
View Alternative Names
DKFZp762A223, FA 4, FA D2, FACD, FACD2_HUMAN, FAD, FANCD, FANCONI ANEMIA COMPLEMENTATION GROUP D, FANCONI PANCYTOPENIA TYPE 4, FLJ23826, Fanconi anemia complementation group D2, Fanconi anemia group D2 protein, OTTHUMP00000158853, OTTHUMP00000207925, Protein FACD2, Type 4 Fanconi pancytopenia
- WB
Lab
Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : FANCD2 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab178705 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.
ab178705 Anti-FANCD2 antibody [EPR2279(3)] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178705 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-FANCD2 antibody [EPR2279(3)] (<a href='/en-us/products/primary-antibodies/fancd2-antibody-epr22793-ab178705'>ab178705</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
FANCD2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human FANCD2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fancd2-knockout-hela-cell-line-ab261743'>ab261743</a>)
Predicted band size: 166 kDa
Observed band size: 166 kDa
false
- WB
Lab
Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : FANCD2 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab108928 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108928 Anti-FANCD2 antibody [EPR2302] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108928 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-FANCD2 antibody [EPR2302] (<a href='/en-us/products/primary-antibodies/fancd2-antibody-epr2302-ab108928'>ab108928</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
FANCD2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human FANCD2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fancd2-knockout-hela-cell-line-ab261743'>ab261743</a>)
Predicted band size: 166 kDa
Observed band size: 166 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human FANCD2 knockout HeLa cell lysate (AB257173)
Homozygous : 4 bp deletion in exon 13
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FANCD2 collaborates with multiple proteins as part of the Fanconi anemia (FA) complex to ensure genomic stability. The FA complex operates by facilitating the repair of DNA interstrand crosslinks that impede replication. FANCD2 becomes activated through monoubiquitination which serves as a signal for recruitment to DNA damage sites. It essentially functions as a coordinator that brings together important components for repair.
Pathways
FANCD2 integrates into the Fanconi anemia pathway and the homologous recombination repair pathway. These pathways are critical for maintaining DNA integrity and preventing chromosomal instability. Within these pathways interactions with proteins such as BRCA1 and BRCA2 reinforce the repair processes. FANCD2's connection to these proteins exemplifies its role in complex mechanisms that preserve genomic fidelity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-FANCD2 antibody [EPR2302] (AB108928)](https://content.abcam.com/products/images/fancd2-antibody-epr2302-ab108928--western-blot-img44207.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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