Human FASTK knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human FASTK knockout HeLa cell lysate (AB263198)
Allele-1 : 20 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human FASTK knockout HeLa cell lysate (AB263198)
Allele-3 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human FASTK knockout HeLa cell lysate (AB263198)
Allele-2 : 13 bp deletion in exon 2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FASTK associates with a larger protein complex involved in mitochondrial RNA surveillance. This complex targets and stabilizes mitochondrial transcripts important for mitochondrial protein synthesis. Through its RNA-binding capability FASTK influences the production of ATP by maintaining proper mitochondrial function and energy metabolism.
Pathways
FASTK operates within mitochondrial gene expression and oxidative phosphorylation pathways. It is associated with proteins like FASTKD2 and TFB2M which also participate in mitochondrial RNA processing and ribosome biogenesis. This interplay is essential for responding to cellular energy demands and maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com