Human FERMT2 (Kindlin 2) knockout HeLa cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human FERMT2 (Kindlin 2) knockout HeLa cell lysate (AB257436)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : FERMT2 knockout HeLa cell lysate (20 μg)
Lane 3 : Saos-2 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265247 (knockout cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Kindlin 2 antibody (<a href='/en-us/products/primary-antibodies/kindlin-2-antibody-ab74030'>ab74030</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
FERMT2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human FERMT2 (Kindlin 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fermt2-kindlin-2-knockout-hela-cell-line-ab265247'>ab265247</a>)
Lane 3:
Saos-2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human FERMT2 (Kindlin 2) knockout HeLa cell lysate (AB257436)
Homozygous : 1 bp deletion in exon 2
Complementary Products
Cell Lines & Lysates
AB265247
Human FERMT2 (Kindlin 2) knockout HeLa cell line
Advanced Validation
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Kindlin-2 contributes to integrin activation and cytoskeletal organization by forming part of a larger complex. This complex stabilizes integrin-talin interactions enhancing the affinity of integrins for their ligands. This function of Kindlin-2 aids in dynamically regulating cell shape migration and mechanical signaling. Its role is significant for normal cellular function and homeostasis because it helps cells to attach and detach when necessary which is important for cell movement and tissue repair.
Pathways
The action of Kindlin-2 integrates with key signaling pathways like the Wnt and PI3K/AKT pathways. In these pathways Kindlin-2 assists in transmitting signals necessary for cellular proliferation and survival. It interacts with other proteins such as talin and integrin β subunits forming a bridge between the cytoskeleton and external signals. This interaction allows cells to adapt to changes in their environment by modulating cell adhesion and migration.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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