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AB258871

Human FGF13 knockout HEK-293T cell lysate

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FGF13 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.

View Alternative Names

FGF-13, FGF13, FHF-2, FHF2, Fibroblast growth factor homologous factor 2, Fibroblast growth factor 13

2 Images
Sanger Sequencing - Human FGF13 knockout HEK-293T cell lysate (AB258871)
  • Sanger seq

Unknown

Sanger Sequencing - Human FGF13 knockout HEK-293T cell lysate (AB258871)

Homozygous : 1 bp insertion in exon4

Western blot - Human FGF13 knockout HEK-293T cell lysate (AB258871)
  • WB

Lab

Western blot - Human FGF13 knockout HEK-293T cell lysate (AB258871)

All lanes:

Western blot - Anti-FGF13 antibody [EPR29561-52] (<a href='/en-us/products/primary-antibodies/fgf13-antibody-epr29561-52-ab325168'>ab325168</a>)

Lane 1:

Wilde-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Lane 2:

Western blot - Human FGF13 knockout HEK-293T cell lysate (ab258871) at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,30 kDa,124 kDa

false

Exposure time: 180s

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.

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Complementary Products

Proteins & Peptides

AB199804

Recombinant Human FGF13 protein

  • 0
  • 0
Primary Antibodies

AB186300

Anti-FGF13 antibody [N235/22]

Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (AB186300)

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  • 0
Cell Lines & Lysates

AB267284

Human FGF13 knockout HEK-293T cell line

Sanger Sequencing - Human FGF13 knockout HEK-293T cell line (AB267284)

  • 0
  • 0
Cell Lines & Lysates

AB258674

Human SLC25A29 knockout HeLa cell lysate

Sanger Sequencing - Human SLC25A29 knockout HeLa cell lysate (AB258674)

  • 0
  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
FGF13
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF13 also known as Fibroblast Growth Factor 13 is a member of the FGF family and plays a significant role in cellular processes. It has an approximate molecular mass of 29 kDa. You often find FGF13 expressed in the brain and heart indicating its involvement in the physiology of these organs. FGF13 does not function as a traditional growth factor through receptors but instead operates intracellularly engaging directly with cellular components.
Biological function summary

FGF13 plays important roles in regulating neuronal development and intracellular signaling pathways. It forms complexes with other proteins modulating their activities within cells. FGF13 is instrumental in neuronal outgrowth and synaptic plasticity essential functions for maintaining a healthy nervous system. Researchers identify FGF13 as important for proper neuronal positioning and survival.

Pathways

FGF13 is intricately involved in signaling mechanisms associated with ion channel regulation and mitogen-activated protein kinase (MAPK) pathway. It interacts with proteins such as kinases and other ion channel components to fine-tune cellular responses to extracellular stimuli. FGF13 by modulating these pathways assists in the precise control of cellular growth differentiation and adaptation.

FGF13 presents a significant connection to neurodevelopmental and cardiac disorders. Mutations in FGF13 or its altered expression levels have links to disorders such as epilepsy and cardiac arrhythmia. Through these diseases FGF13 often interacts with proteins like ion channels affecting their function and stability. These interactions highlight the potential of FGF13 as a target for therapeutic interventions in related disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com