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AB259781

Human FGFR3 knockout HEK-293 cell lysate

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FGFR3 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99.28%.
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Western blot - Human FGFR3 knockout HEK-293 cell lysate (AB259781)
  • WB

Lab

Western blot - Human FGFR3 knockout HEK-293 cell lysate (AB259781)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2 : FGFR3 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab133644 observed at 125kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133644 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in FGFR3 knockout cell line ab259775 (knockout cell lysate ab259781). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and FGFR3 knockout samples were subjected to SDS-PAGE. ab133644 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (<a href='/en-us/products/primary-antibodies/fgfr3-antibody-epr23043-ab133644'>ab133644</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

FGFR3 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human FGFR3 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-fgfr3-knockout-hek-293-cell-line-ab259775'>ab259775</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 76 kDa,88 kDa

Observed band size: 125 kDa

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Next Generation Sequencing - Human FGFR3 knockout HEK-293 cell lysate (AB259781)
  • NGS

Supplier Data

Next Generation Sequencing - Human FGFR3 knockout HEK-293 cell lysate (AB259781)

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99.28%

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99.28%

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Complementary Products

Primary Antibodies

AB133644

Anti-FGFR3 antibody [EPR2304(3)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

  • 5
  • 2
Cell Lines & Lysates

AB259775

Human FGFR3 knockout HEK-293 cell line

Advanced Validation

Western blot - Human FGFR3 knockout HEK-293 cell line (AB259775)

  • 0
  • 0
Proteins & Peptides

AB290073

Recombinant Human FGFR3 Protein (Active)

Premium Bioactive

Functional Studies - Recombinant Human FGFR3 Protein (Active) (AB290073)

  • 0
  • 0
Primary Antibodies

AB76464

Anti-FGFR1 antibody [EPR806Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-FGFR1 antibody [EPR806Y] (AB76464)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259781-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human FGFR3 knockout HEK293 cell lysate", "number":"AB259781-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK-293 cell lysate", "number":"AB259781-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
FGFR3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.
Biological function summary

FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.

Pathways

FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.

FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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