FKBP10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Alternative names
65 kDa FK506 binding protein, FK506 binding protein 10, FK506 binding protein 10 65 kDa, FKBP 65, Immunophilin FKBP65, PPIase, Rotamase, hFKBP 65, peptidyl-prolyl cis-trans isomerase
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FKBP10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
- Concentration
Properties
- Gene name
- FKBP10
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FKBP10 also known as FKBP65 is a protein that functions as a peptidyl-prolyl cis-trans isomerase (PPIase). This protein with a mass of approximately 65 kDa is involved in protein folding by catalyzing the isomerization of proline residues in polypeptide chains. FKBP10 is expressed in the endoplasmic reticulum of cells and it contributes to the synthesis and maturation of collagens which are essential for the structural integrity of various tissues.
- Biological function summary
FKBP10 plays a critical role in ensuring proper collagen biosynthesis. It forms part of a complex involved in stabilizing the collagen triple-helix structure during its assembly in the endoplasmic reticulum. This action by FKBP10 is necessary for maintaining tissue strength and flexibility due to its involvement in cross-linking collagen fibers. This activity highlights the importance of FKBP10 in connective tissue development and maintenance.
- Pathways
FKBP10 is deeply embedded in the collagen synthesis and folding processes. It interacts within the pathways that incorporate transforming growth factor-beta (TGF-β) influencing extracellular matrix formation and maintenance. FKBP10's function is linked to proteins like prolyl 4-hydroxylase which are vital for collagen modification impacting the maturation and structural stability of collagen fibrils.
- Associated diseases and disorders
FKBP10 mutations have associations with connective tissue disorders such as osteogenesis imperfecta and Ehlers-Danlos syndrome. These diseases result from defects in collagen formation and stability. FKBP10's dysfunction can lead to compromised structural properties of tissues correlating with irregularities linked to the protein HSP47 another chaperone involved in collagen processing. Understanding FKBP10's role in these diseases aids in identifying potential therapeutic targets for correcting defects in collagen biosynthesis.
Product promise
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3 product images
Sanger Sequencing - Human FKBP10 knockout HEK-293T cell lysate (ab258872)
Allele-2: Insertion of the selection cassette in exon 1
Sanger Sequencing - Human FKBP10 knockout HEK-293T cell lysate (ab258872)
Allele-1: Insertion of the selection cassette in exon 1
Sanger Sequencing - Human FKBP10 knockout HEK-293T cell lysate (ab258872)
Allele-3: 23 bp deletion in exon 1
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- Visit the general protocols
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