FLAD1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Alternative names
A930017E24Rik, EC 2.7.7.2, FAD 1, FAD pyrophosphorylase, FAD synthetase, FAD synthetase region, FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae), FAD1, S. cerevisiae, homolog of, FADS, FMN adenylyl transferase, Fad1, flavin adenine dinucleotide synthetase, homolog, Fad1, flavin adenine dinucleotide synthetase, homolog (yeast), Flad1 protein, Flavin adenine dinucleotide synthetase, Flavin adenine dinucleotide synthetase, S. cerevisiae, homolog of, MGC187714, MGC31803, MGC40255, Molybdenum cofactor biosynthesis protein-like region, OTTHUMP00000035547, OTTHUMP00000035548, Pp591, RFad1, flavin adenine dinucleotide synthetase, homolog (yeast), RP11-307C12.7
What's included?
Recommended products
FLAD1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
- Concentration
Properties
- Gene name
- FLAD1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FLAD1 also known as FAD synthase is an enzyme that plays a role mechanically in the synthesis of Flavin Adenine Dinucleotide (FAD) from ATP and FMN (Flavin Mononucleotide). FLAD1 has an approximate mass of 66 kDa. It is expressed in a variety of tissues with high levels in liver heart and kidneys. Its primary responsibility is to ensure the sufficient production of FAD necessary for various metabolic enzymes and biological processes.
- Biological function summary
FLAD1 facilitates essential cellular processes by enabling the synthesis of FAD a cofactors required for the functioning of oxidoreductase enzymes involved in redox reactions. FAD forms part of several enzyme complexes vital for energy production and cellular respiration including those complexes located within the mitochondria. FLAD1's action allows cells to maintain metabolic activities such as the Krebs cycle and the electron transport chain.
- Pathways
Flavin Adenine Dinucleotide produced by FLAD1 is fundamental for proper mitochondrial function through participation in the oxidative phosphorylation and the beta-oxidation pathways. These pathways are critical for efficient ATP production. Related proteins that also contribute to these pathways include succinate dehydrogenase and acyl-CoA dehydrogenase which depend on FAD to perform their metabolic roles.
- Associated diseases and disorders
Disruptions in FLAD1 function can contribute to metabolic-related conditions. The dysfunctional production of FAD can associate with mitochondrial disorders where energy production is impaired and FLAD1 mutations are linked to conditions like multiple acyl-CoA dehydrogenase deficiency (MADD). Proteins such as ETFDH another enzyme involved in the electron transport chain have connections with FLAD1 especially in these metabolic disorders highlighting the enzyme's importance in maintaining normal cellular function.
Product promise
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1 product image
Sanger Sequencing - Human FLAD1 knockout HEK-293T cell lysate (ab258873)
Homozygous: 1 bp insertion in exon 3
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