Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate
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- WB
Lab
Western blot - Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate (AB257442)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : FLOT2 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : A549 cell lysate (20 ug)
ab181988 was shown to specifically react with Flotillin 2/ESA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266847 (knockout cell lysate ab257442) was used. Wild-type and Flotillin 2/ESA knockout samples were subjected to SDS-PAGE. ab181988 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (<a href='/en-us/products/primary-antibodies/flotillin-2-esa-antibody-epr14128b-ab181988'>ab181988</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
FLOT2 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-flot2-flotillin-2-esa-knockout-hek-293t-cell-line-ab266847'>ab266847</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
A549 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 27 kDa,47 kDa
Observed band size: 27 kDa,47 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human FLOT2 (Flotillin 2/ESA) knockout HEK-293T cell lysate (AB257442)
Homozygous : 5 bp deletion in exon4
Complementary Products
Primary Antibodies
AB181988
Anti-Flotillin 2/ESA antibody [EPR14128(B)]
RabMAb|Recombinant|KO Validated
![Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (AB181988)](https://content.abcam.com/products/images/flotillin-2-esa-antibody-epr14128b-ab181988--immunoprecipitation-img67184.jpg)
- 5
- 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Flotillins serve as scaffolding proteins in cellular signaling and play a part in the regulation of endocytosis. Flotillin 2 often forms a hetero-oligomeric complex with Flotillin 1 facilitating its role in organizing membrane microdomains. This interaction supports cellular activities like molecular transport and signal relay. Flotillin 2 contributes to dynamic cellular structures and has been suggested to influence actin cytoskeleton assembly giving it a role in cellular morphology and motility.
Pathways
Flotillin 2 associates with important cell signaling pathways notably the MAPK and Wnt signaling pathways. In MAPK pathways flotillin 2 influences cell proliferation and differentiation by modulating interaction with signaling proteins like Ras and ERK. In the context of the Wnt signaling pathway it impacts cellular communication influencing gene expression and development processes by engaging with Dishevelled proteins. These pathways highlight its critical part in processes like cellular growth and communication.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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