Human FOCAD (Focadhesin) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
FOCAD KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon12.
View Alternative Names
FOCAD_HUMAN, Focadhesin, KIAA1797
- Sanger seq
Unknown
Sanger Sequencing - Human FOCAD (Focadhesin) knockout HeLa cell lysate (AB263203)
Homozygous : 1 bp deletion in exon12
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Focadhesin contributes to cellular adhesion and communication mechanisms. It operates as part of a multiprotein complex associated with adhesion molecules such as cadherins and integrins. This complex facilitates the stabilization of cell junctions supporting structural cohesion between cells. Focadhesin's presence is necessary for processes involving epithelial cell cohesion and barrier function. Its interactions with other adhesion molecules promote the assembly and maintenance of intercellular connections.
Pathways
Focadhesin plays a significant role in cell signaling pathways that control cell adhesion and migration particularly the Wnt and PI3K/AKT pathways. Its interaction with proteins like beta-catenin and phosphoinositide 3-kinase highlights its importance in signaling cascades that affect cellular dynamics and growth. These pathways influence cellular responses and integrity impacting processes like tissue remodeling and stress responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com