Human FOXA1 knockout HeLa cell lysate

• WB

Lab

Western blot - Human FOXA1 knockout HeLa cell lysate (AB256920)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : FOXA1 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab55178 observed at 49 kDa. Red - loading control ab181602 observed at 37 kDa.
ab55178 Anti-FOXA1 antibody was shown to specifically react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261823 (knockout cell lysate ab256920) was used. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab55178 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4^°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
HeLa cells are predicted to express FOXA1, with no expression of FOXA2 or FOXA3.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 + FOXA3 antibody [1B1] (<a href='/en-us/products/primary-antibodies/foxa1-antibody-1b1-ab55178'>ab55178</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FOXA1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FOXA1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-foxa1-knockout-hela-cell-line-ab261823'>ab261823</a>)

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

• WB

Lab

Western blot - Human FOXA1 knockout HeLa cell lysate (AB256920)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : FOXA1 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab170933 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab170933 Anti-FOXA1 antibody [EPR10881] was shown to specifically react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261823 (knockout cell lysate ab256920) was used. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170933 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Hela cells are predicted to express FOXA1, not FOXA2 and FOXA3

All lanes:

Western blot - Anti-FOXA1 + FOXA2 + FOXA3 antibody [EPR10881] (<a href='/en-us/products/primary-antibodies/foxa1-antibody-epr10881-ab170933'>ab170933</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FOXA1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FOXA1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-foxa1-knockout-hela-cell-line-ab261823'>ab261823</a>)

Predicted band size: 49 kDa

Observed band size: 52 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human FOXA1 knockout HeLa cell lysate (AB256920)

Allele-2 : 4 bp insertion in exon2

• Sanger seq

Unknown

Sanger Sequencing - Human FOXA1 knockout HeLa cell lysate (AB256920)

Allele-1 : 1 bp insertion in exon2