JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257444

Human FSCN1 (Fascin) knockout HCT116 cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

FSCN1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

View Alternative Names

55 kDa actin-bundling protein, Actin bundling protein, FLJ38511, FSCN1_HUMAN, Fascin, Fascin 1, Fascin actin bundling protein 1, Fascin homolog 1, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus), Fascin, sea urchin, homolog of, 1, HSN, SNL, Singed (Drosophila) like (sea urchin fascin homolog like), Singed drosophila homolog like, Singed like (fascin homolog sea urchin), Singed like (fascin homolog sea urchin) (Drosophila), Singed, drosophila, homolog of, Singed-like protein, Strongylocentrotus purpuratus, actin bundling protein, 55-KD, p55

3 Images
Western blot - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)
  • WB

Lab

Western blot - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)

Lane 1 : Wild-type HCT116 cell lysate (20 ug)
Lane 2 : FSCN1 knockout HCT116 cell lysate (20 ug)
Lane 3 : SH-SY5Y cell lysate (20 ug)
Lane 4 : K-562 cell lysate (20 ug)

ab126772 was shown to specifically react with Fascin in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266895 (knockout cell lysate ab257444) was used. Wild-type and Fascin knockout samples were subjected to SDS-PAGE. ab126772 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Fascin antibody [EP5902] (<a href='/en-us/products/primary-antibodies/fascin-antibody-ep5902-ab126772'>ab126772</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

FSCN1 knockout HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human FSCN1 (Fascin) knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-fscn1-fascin-knockout-hct116-cell-line-ab266895'>ab266895</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 54 kDa

Observed band size: 60 kDa

false

Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)
  • Sanger seq

Lab

Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)

Allele-2 : Insertion of the selection cassette in exon1

Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)
  • Sanger seq

Unknown

Sanger Sequencing - Human FSCN1 (Fascin) knockout HCT116 cell lysate (AB257444)

Allele-1 : 1 bp deletion in exon1

Key facts

Cell type

HCT116

Species or organism

Human

Tissue

Colon

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

Disease

Carcinoma

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257444-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human FSCN1 knockout HCT116 cell lysate", "number":"AB257444-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257444-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
FSCN1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB126772

Anti-Fascin antibody [EP5902]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Fascin antibody [EP5902] (AB126772)

  • 0
  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
  • 0
Primary Antibodies

AB216347

Anti-SNAIL antibody [EPR21043]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-SNAIL antibody [EPR21043] (AB216347)

  • 1
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com