Human FUCA2 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human FUCA2 knockout HEK-293T cell lysate (AB263205)
Homozygous : 2 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FUCA2 participates in the degradation of fucosylated glycoconjugates. It does not form part of a larger complex; however it operates in concert with other glycosidases to ensure efficient turnover of glycoproteins and glycolipids. This turnover is important in maintaining cellular and systemic homeostasis influencing processes like immune response and inflammatory regulation.
Pathways
FUCA2 is involved in the lysosomal degradation pathway and also interacts within the glycosphingolipid metabolism pathway. It cooperates with proteins like alpha-L-fucosidase 1 (FUCA1) to facilitate the catabolism of glycostructures. These metabolic pathways are essential for recycling cellular materials and processing complex sugar molecules highlighting their role in maintaining cellular efficiency.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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