Human FUK knockout HEK-293T cell lysate
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FCSK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 11 and 1 bp deletion in exon 11 and 25 bp deletion in exon 11.
View Alternative Names
1110046B12Rik, FLJ39408, FUK_HUMAN, Fucokinase, L-fucose kinase
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell lysate (AB263206)
Allele-3 : 1 bp deletion in exon11
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell lysate (AB263206)
Allele-1 : 25 bp deletion in exon11
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell lysate (AB263206)
Allele-2 : 10 bp deletion in exon11
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FUK plays a role in the salvage pathway of L-fucose metabolism. This pathway recycles free L-fucose into GDP-fucose which becomes essential for glycoprotein and glycolipid synthesis. FUK operates not as part of a larger protein complex but individually facilitating the utilization of fucose derived from dietary sources or breakdown of glycoproteins. The enzyme regulates intracellular concentrations of fucose impacting cell surface glycoconjugates important for cell signaling.
Pathways
FUK integrates into the fucose salvage pathway that links to glycosylation processes. This situates FUK within the larger context of glycan biosynthesis pathways where it intersects with enzymes such as GDP-fucose synthase. These pathways significantly influence the modification and function of proteins and lipids on cell surfaces affecting cellular communication and host-pathogen interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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