Human FXR1 knockout HeLa cell lysate
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FXR1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9.
View Alternative Names
FXR1P, FXR1_HUMAN, Fragile X mental retardation autosomal homolog 1, Fragile X mental retardation syndrome-related protein 1, hFXR1p
- WB
Lab
Western blot - Human FXR1 knockout HeLa cell lysate (AB264505)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : FXR1 knockout HeLa cell lysate (20 ug)
Lane 3 : Hep G2 cell lysate (20 ug)
Lane 4 : A549 cell lysate (20 ug)
Lane 5 : HEK-293 cell lysate (20 ug)
Lanes 1 - 5 : Merged signal (red and green). Green - ab129089 observed at 70-80 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab129089 was shown to react with FXR1 in wild-type HeLa cells in Western blot with loss of signal observed in FXR1 knockout cell line ab264017 (knockout cell lysate ab264505). Wild-type HeLa and FXR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab129089 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-FXR1 antibody [EPR7932] (<a href='/en-us/products/primary-antibodies/fxr1-antibody-epr7932-ab129089'>ab129089</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
FXR1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human FXR1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fxr1-knockout-hela-cell-line-ab264017'>ab264017</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
A549 cell lysate at 20 µg
Lane 5:
HEK-293 cell lysate at 20 µg
Predicted band size: 70 kDa
Observed band size: 70-80 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FXR1 is involved in the post-transcriptional regulation of gene expression important for normal cell function and development. It forms a complex with FMRP playing an important role in the transport and translation of specific subsets of mRNAs in neurons. This interaction suggests it contributes significantly to the synaptic plasticity mechanisms influencing learning and memory. Moreover FXR1 also seems to have functions in cell proliferation and muscle differentiation.
Pathways
FXR1 participates in key regulatory pathways of protein synthesis and neuronal communication. It is significant in the mTOR signaling pathway where it potentially interacts with other proteins like FXR2 and FMR1 to modulate translational control. FXR1's involvement in this pathway suggests a role in cellular growth and neuron-specific processes affecting how cells respond to various growth signals by altering protein synthesis rates.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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