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FYN KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 14 bp deletion Frameshift: 100%.

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Images

Western blot - Human FYN knockout HEK-293 cell lysate (AB272440), expandable thumbnail
  • Western blot - Human FYN knockout HEK-293 cell lysate (AB272440), expandable thumbnail
  • Next Generation Sequencing - Human FYN knockout HEK-293 cell lysate (AB272440), expandable thumbnail

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing, Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 14 bp deletion Frameshift: 100%

Alternative names

What's included?

1 Kit
Components
Human FYN knockout HEK-293 cell lysate
1 x 100 µg
Human wild-type HEK-293 cell lysate
1 x 100 µg

Recommended products

FYN KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 14 bp deletion Frameshift: 100%.

Key facts

Cell type

HEK-293

Mutation description

Knockout achieved by CRISPR/Cas9 X = 14 bp deletion Frameshift: 100%

Concentration
Loading...

Properties

Gene name

FYN

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Next Generation Sequencing, Western blot

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension
Adherent
Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes

Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Fyn is a Src family tyrosine kinase with a molecular weight of approximately 59 kDa. It is often referred to as Fyn kinase and plays a significant role in signal transduction processes. This protein is observed in various tissues with high expression in the brain T cells and platelets. Fyn kinase is known for its function in cell communication and regulates different cellular processes in these tissues.

Biological function summary

Fyn kinase influences cell growth differentiation and survival. It is a part of larger signaling complexes including those accountable for the development and function of the central nervous system. The protein's activity is important for neural plasticity and supports synaptic communication particularly within the brain. Fyn can interact with other proteins in these complexes to help direct cellular responses to external signals.

Pathways

Fyn kinase is central to several intracellular signaling pathways such as the T cell receptor (TCR) signaling pathway and the integrin signaling pathway. The TCR signaling pathway involves the regulation of immune responses while the integrin signaling pathway contributes to cell adhesion and migration. Other proteins like Lck and Yes interact with Fyn within these pathways adding another layer of regulation and response in various cell types.

Associated diseases and disorders

Fyn kinase has associations with Alzheimer's disease and certain types of cancer. In Alzheimer's dysregulation of Fyn activity contributes to synaptic dysfunction and neuronal damage. In cancer aberrant Fyn signaling can affect cellular proliferation and metastasis. Proteins such as Tau in Alzheimer's disease and Src in cancer relate to Fyn through their respective pathological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Human FYN knockout HEK-293 cell lysate (ab272440), expandable thumbnail

    Western blot - Human FYN knockout HEK-293 cell lysate (ab272440)

    Lane 1: Wild-type HEK-293 cell lysate 20 μgLane 2: FYN knockout HEK-293 cell lysate 20 μgFalse colour image of Western blot: Anti-Fyn antibody [EPR19636] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Fyn antibody [EPR19636] ab184276 was shown to bind specifically to Fyn. A band was observed at 60 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in FYN knockout cell line Human FYN knockout HEK-293 cell line ab269630 (knockout cell lysate ab272440). To generate this image, wild-type and FYN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Fyn antibody [EPR19636] (Anti-Fyn antibody [EPR19636] ab184276) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: FYN knockout HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Western blot - Human FYN knockout HEK-293 cell lysate (ab272440), expandable thumbnail

    Western blot - Human FYN knockout HEK-293 cell lysate (ab272440)

    Lane 1: Wild-type HEK-293 cell lysate 20 μgLane 2: FYN knockout HEK-293 cell lysate 20 μgFalse colour image of Western blot: Anti-Fyn antibody [EPR5500] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Fyn antibody [EPR5500] ab125016 was shown to bind specifically to Fyn. A band was observed at 60 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in FYN knockout cell line Human FYN knockout HEK-293 cell line ab269630 (knockout cell lysate ab272440). To generate this image, wild-type and FYN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-Fyn antibody [EPR5500] (Anti-Fyn antibody [EPR5500] ab125016) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: FYN knockout HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 60 kDa

    Observed band size: 60 kDa

  • Next Generation Sequencing - Human FYN knockout HEK-293 cell lysate (ab272440), expandable thumbnail

    Next Generation Sequencing - Human FYN knockout HEK-293 cell lysate (ab272440)

    Knockout achieved by CRISPR/Cas9; X = 14 bp deletion; Frameshift: 100%

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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