Human FZD1 (Frizzled homolog 1) knockout HeLa cell lysate
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FZD1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.
View Alternative Names
Chfz-1, DKFZp564G072, Dfrizzled-1, FLJ95923, FZD1_HUMAN, Frizzled homolog 1, Frizzled homolog 1 (Drosophila), Frizzled-1, Fz receptor 1, Fz-1, FzE1, Rfz1, Wnt receptor, Xfrizzled-1, hFz1
- Sanger seq
Unknown
Sanger Sequencing - Human FZD1 (Frizzled homolog 1) knockout HeLa cell lysate (AB257956)
Homozygous : 2 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FZD1 participates in important processes concerning cell development differentiation and growth. As a member of the Frizzled family FZD1 often forms a functional complex with LRP5/6 co-receptors upon Wnt ligand binding. This complex initiates intracellular signaling cascades that lead to the regulation of target genes. In addition to its role in development FZD1 also has a major impact on maintaining stem cell renewal and tissue homeostasis.
Pathways
FZD1 functions significantly in both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway FZD1 interacts with proteins such as Disheveled (DVL) and β-catenin leading to alterations in gene expression that influence cellular proliferation and differentiation. In the non-canonical pathway FZD1 also associates with other proteins including Rho and JNK which contribute to changes in cell polarity and motility. These pathways highlight FZD1's involvement in complex networks essential for cellular and tissue dynamics.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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