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AB258874

Human FZD6 (Frizzled 6) knockout HeLa cell lysate

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FZD6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3 and 5 bp deletion in exon3.

View Alternative Names

FZD6_HUMAN, Frizzled 6 seven transmembrane spanning receptor, Frizzled drosophila homolog of 6, Frizzled family receptor 6, Frizzled homolog 6, Frizzled homolog 6 (Drosophila), Frizzled-6, Fz-6, Hfz 6, NDNC10

4 Images
Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)
  • WB

Lab

Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)

Lane 1 : Wild-type HeLa cell lysate 30 µg

Lane 2 : FZD6 knockout HeLa cell lysate 30 µg

Lane 3 : HTC116 cell lysate 30 µg

Lane 4 : Daudi cell lysate 30 µg

Lanes 1 - 4 : Merged signal (red and green). Green - anti-Frizzled 6 antibody observed at 70 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

Anti-Frizzled 6 antibody was shown to react with Frizzled 6 in wild-type cells in Western blot with loss of signal observed in FZD6 knockout cell line ab265280 (FZD6 knockout cell lysate ab258874). Wild-type and FZD6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with anti-Frizzled 6 antibody and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Anti-Frizzled 6 antibody at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 30 µg

Lane 2:

Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fzd6-frizzled-6-knockout-hela-cell-line-ab265280'>ab265280</a>)

Lane 2:

Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (ab258874)

Lane 2:

FZD6 knockout HeLa cell lysate at 30 µg

Lane 3:

HTC116 cell lysate at 30 µg

Lane 4:

Daudi cell lysate at 30 µg

Secondary

Lanes 1 - 4:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 4:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Observed band size: 70 kDa

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Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)
  • Sanger seq

Unknown

Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)

Allele-2 : 2 bp deletion in exon3

Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)
  • Sanger seq

Unknown

Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)

Allele-3 : 1 bp deletion in exon3

Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)
  • Sanger seq

Unknown

Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (AB258874)

Allele-1 : 5 bp deletion in exon3

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3 and 5 bp deletion in exon3.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
FZD6
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Frizzled 6 also known as FZD6 is a receptor encoded by the FZD6 gene that plays an important role in Wnt signaling. Frizzled 6 is a transmembrane protein part of the Frizzled family which typically contains seven transmembrane domains. It has a molecular mass of approximately 75 kDa. Expression of Frizzled 6 occurs in various tissues including the brain lung pancreas and kidney highlighting its diverse functional significance within the body.
Biological function summary

Frizzled 6 acts as a receptor in the Wnt signaling pathway which is important in cellular communication and regulation. It does not function alone; it is part of a complex network involving other Frizzled proteins and Wnt ligands that are key for processes such as cell proliferation differentiation and migration. This signaling is important for maintaining proper cellular responses and is integral to developmental processes.

Pathways

Frizzled 6 integrates into the non-canonical Wnt signaling pathway which diverges from the canonical pathway by not involving β-catenin. In this pathway it cooperates with key proteins like Disheveled (DVL) and forms complexes with Wnt ligands to elicit specific cellular responses. This engagement is essential for tailoring responses to external cellular cues especially in processes like tissue polarity and neural tube closure during embryonic development.

Frizzled 6 relates to diseases such as neural tube defects and some forms of cancer. Alterations in Frizzled 6 expression or function can interfere with Wnt signaling integrity leading to such conditions. In these disease states interactions with Wnt ligands and other proteins involved in this signaling pathway including Disheveled underline the receptor’s importance and the risk related to its dysregulation. Understanding Frizzled 6's involvement could lead to targeted therapeutic approaches for these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB290728

Anti-Frizzled 6 antibody [EPR25319-149]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 6 antibody [EPR25319-149] (AB290728)

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Proteins & Peptides

AB276698

Recombinant Human Frizzled 6 protein (His tag)

SDS-PAGE - Recombinant Human Frizzled 6 protein (His tag) (AB276698)

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Cell Lines & Lysates

AB265280

Human FZD6 (Frizzled 6) knockout HeLa cell line

Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell line (AB265280)

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  • 0

Product promise

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