FZR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon9.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon9.
Alternative names
CDC20 like 1b, CDC20-like protein 1, CDC20C, CDH1, Cdh1/Hct1 homolog, FYR, FZR 2, FZR_HUMAN, Fizzy related protein, Fizzy related protein 1, Fizzy-related protein homolog, Fizzy/cell division cycle 20 related 1, Fzr, Fzr1 protein, HCDH 1, KIAA1242
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FZR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon9.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon9.
- Concentration
Properties
- Gene name
- FZR1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FZR1 also known as fizzy-related protein homolog plays a critical mechanical role in cell cycle regulation as a co-activator of the anaphase-promoting complex/cyclosome (APC/C). The FZR1 protein has a mass of about 83 kDa. It is involved in the transition from metaphase to anaphase during cell division. This protein expresses in various tissues but shows higher levels in proliferating cells due to its role in cell cycle control.
- Biological function summary
FZR1 helps regulate the degradation of cell cycle proteins by tagging them for destruction in a process involving the ubiquitin-proteasome system. FZR1 forms a complex with APC/C pushing the progression of the cell cycle and ensuring proper chromosome segregation. This activity prevents genomic instability by maintaining the correct order of cell cycle phases.
- Pathways
Researchers place FZR1 in the cell cycle pathway particularly in regulating the mitotic exit and G1/S transition. One significant pathway involves its partnership with the cyclin B1 protein where FZR1 aids in its ubiquitination and subsequent degradation. It is also related to pathways involving the retinoblastoma protein (Rb) which adds a layer of regulation over the cycle and ensures DNA replication precision.
- Associated diseases and disorders
FZR1 associates with cancer due to its involvement in controlling cell division and preventing aneuploidy. Disruption in FZR1 function can lead to misregulated cell growth which is often observed in tumors. Furthermore some studies connect FZR1 to neurodegenerative disorders notably through its interactions with proteins in the nervous system although these connections are still under investigation.
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2 product images
Western blot - Human FZR1 knockout HEK-293T cell lysate (ab263208)
Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2:FZR1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3:HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
Lane 4:A431 (Human epidermoid carcinoma cell line) whole cell lysate (20 ug)Anti-FZR1 antibody [AR38.2] ab77885 was shown to specifically react with FZR1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human FZR1 knockout HEK-293T cell line ab267263 (knockout cell lysate ab263208) was used. Wild-type and FZR1 knockout samples were subjected to SDS-PAGE. Anti-FZR1 antibody [AR38.2] ab77885 and Anti-GAPDH antibody[EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FZR1 antibody [AR38.2] (Anti-FZR1 antibody [AR38.2] ab77885) at 1/500 dilution
Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: FZR1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: Western blot - Human FZR1 knockout HEK-293T cell line (Human FZR1 knockout HEK-293T cell line ab267263)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Sanger Sequencing - Human FZR1 knockout HEK-293T cell lysate (ab263208)
Homozygous: 1 bp insertion in exon9
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