GAS6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%.
Images
Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%
Alternative names
AXL receptor tyrosine kinase ligand, AXL stimulatory factor, AXLLG, AXS F, DKFZp666G247, FLJ34709, GAS6_HUMAN, Growth arrest specific 6, Growth-arrest-specific protein 6, Growth-arrest-specific protein 6 precursor
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GAS6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%.
Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%
- Concentration
Properties
- Gene name
- GAS6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Gas6 also known as growth arrest-specific 6 weighs approximately 75 kDa and functions mechanically as a ligand for the TAM (Tyro3 Axl and Mer) receptor tyrosine kinases. Gas6 protein facilitates cell signaling by binding especially to AXL receptor initiating various downstream cellular responses. It is widely expressed in different tissues including the nervous system cardiovascular system and kidneys. Additionally its expression increases in response to cellular stress and inflammatory stimuli.
- Biological function summary
Gas6 influences several cellular processes such as cell survival proliferation and migration. Gas6 exerts its effects in a complex with AXL receptor which is part of a larger network involving other TAM receptors. By activating AXL Gas6 modulates immune responses inflammation and tissue repair. The protein also plays a significant role in phagocytosis helping remove apoptotic cells thereby maintaining tissue homeostasis.
- Pathways
The Gas6 protein is significantly engaged in the PI3K/AKT signaling pathway important for promoting cell survival and inhibiting apoptosis. Additionally Gas6 interacts with the NF-kB pathway which regulates inflammation and immune responses. Both pathways highlight the synergy between Gas6 and AXL emphasizing their contribution to cellular integrity and response to injury.
- Associated diseases and disorders
Gas6's dysregulation associates with cancer and atherosclerosis. In oncogenesis altered Gas6-AXL signaling promotes tumor progression and resistance to therapy implicating it in diverse cancers. Meanwhile in cardiovascular diseases elevated Gas6 levels correlate with atherosclerosis interacting with inflammatory proteins such as interleukins. Understanding Gas6's role in these conditions offers potential for therapeutic targeting and biomarker development.
Product promise
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1 product image
Western blot - Human GAS6 knockout HEK-293 cell lysate (ab275811)
Lane 1: Wild-type HEK-293 cell lysate 20 μg
Lane 2: GAS6 knockout HEK-293 cell lysate 20 μg
Lane 3: THP-1 cell lysate 20 μg
False colour image of Western blot: Anti-GAS 6 antibody [EPR23931-27] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-GAS 6 antibody [EPR23931-27] ab264098 was shown to bind specifically to GAS 6. A band was observed at 75 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in GAS6 knockout cell line Human GAS6 knockout HEK-293 cell line ab275837 (knockout cell lysate ab275811). To generate this image, wild-type and GAS6 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-GAS 6 antibody [EPR23931-27] (Anti-GAS 6 antibody [EPR23931-27] ab264098) at 1/1000 dilution
Lane 1: Wild-type HEK-293 cell lysate at 20 µg
Lane 2: GAS6 knockout HEK-293 cell lysate at 20 µg
Lane 2: Western blot - Human GAS6 knockout HEK-293 cell line (Human GAS6 knockout HEK-293 cell line ab275837)
Lane 3: THP-1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 75 kDa
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