Human GAS6 knockout HEK-293 cell lysate
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GAS6 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%.
View Alternative Names
AXL receptor tyrosine kinase ligand, AXL stimulatory factor, AXLLG, AXS F, DKFZp666G247, FLJ34709, GAS6_HUMAN, Growth arrest specific 6, Growth-arrest-specific protein 6, Growth-arrest-specific protein 6 precursor
- WB
Lab
Western blot - Human GAS6 knockout HEK-293 cell lysate (AB275811)
Lane 1 : Wild-type HEK-293 cell lysate 20 μg
Lane 2 : GAS6 knockout HEK-293 cell lysate 20 μg
Lane 3 : THP-1 cell lysate 20 μg
False colour image of Western blot : Anti-GAS 6 antibody [EPR23931-27] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab264098 was shown to bind specifically to GAS 6. A band was observed at 75 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in GAS6 knockout cell line ab275837 (knockout cell lysate ab275811). To generate this image, wild-type and GAS6 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-GAS 6 antibody [EPR23931-27] (<a href='/en-us/products/primary-antibodies/gas-6-antibody-epr23931-27-ab264098'>ab264098</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 cell lysate at 20 µg
Lane 2:
GAS6 knockout HEK-293 cell lysate at 20 µg
Lane 2:
Western blot - Human GAS6 knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-gas6-knockout-hek-293-cell-line-ab275837'>ab275837</a>)
Lane 3:
THP-1 cell lysate at 20 µg
Predicted band size: 80 kDa
Observed band size: 75 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Gas6 influences several cellular processes such as cell survival proliferation and migration. Gas6 exerts its effects in a complex with AXL receptor which is part of a larger network involving other TAM receptors. By activating AXL Gas6 modulates immune responses inflammation and tissue repair. The protein also plays a significant role in phagocytosis helping remove apoptotic cells thereby maintaining tissue homeostasis.
Pathways
The Gas6 protein is significantly engaged in the PI3K/AKT signaling pathway important for promoting cell survival and inhibiting apoptosis. Additionally Gas6 interacts with the NF-kB pathway which regulates inflammation and immune responses. Both pathways highlight the synergy between Gas6 and AXL emphasizing their contribution to cellular integrity and response to injury.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB278034
Anti-GAS 6 antibody [EPR22812-36] - BSA and Azide free (Detector)
RabMAb|Recombinant
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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