Human GBA knockout HeLa cell lysate
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GBA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
View Alternative Names
Acid beta-glucosidase, Alglucerase, BETA GLUCOSIDASE, ACID, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, EC 3.2.1.45, GBA1, GC-B, GCase, GLCM_HUMAN, GLUCOCEREBROSIDASE PSEUDOGENE, Gba protein, Glucocerebrosidase, Glucocerebrosidase (alt.), Glucosidase beta, Glucosidase, beta, acid, Glucosidase, beta; acid (includes glucosylceramidase), Glucosylceramidase, Imiglucerase, Lysosomal glucocerebrosidase, OTTHUMP00000033992, OTTHUMP00000033993, betaGC
- WB
Lab
Western blot - Human GBA knockout HeLa cell lysate (AB256929)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : GBA knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab128879 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.
ab128879 Anti-GBA antibody [EPR5143(3)] was shown to specifically react with GBA in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265038 (knockout cell lysate ab256929) was used. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab128879 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GBA antibody [EPR5143(3)] (<a href='/en-us/products/primary-antibodies/gba-antibody-epr51433-ab128879'>ab128879</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GBA knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GBA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gba-knockout-hela-cell-line-ab265038'>ab265038</a>)
Predicted band size: 60 kDa
Observed band size: 60 kDa
false
- WB
Lab
Western blot - Human GBA knockout HeLa cell lysate (AB256929)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : GBA knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab125065 observed at 70 kDa. Red - loading control ab8245 observed at 37 kDa.
ab125065 Anti-GBA antibody [EPR5142] was shown to specifically react with GBA in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265038 (knockout cell lysate ab256929) was used. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab125065 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GBA antibody [EPR5142] (<a href='/en-us/products/primary-antibodies/gba-antibody-epr5142-ab125065'>ab125065</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GBA knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GBA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gba-knockout-hela-cell-line-ab265038'>ab265038</a>)
Predicted band size: 60 kDa
Observed band size: 70 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GBA knockout HeLa cell lysate (AB256929)
Homozygous : 1 bp insertion in exon 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GBA plays an important role in sphingolipid metabolism. It participates in the degradation of glycolipids within the lysosome contributing to lipid recycling. It acts independently rather than as a part of a major enzymatic complex. Through its role in degrading glucosylceramide GBA influences cellular homeostasis and bioenergetics ensuring balance in neural and systemic lipid levels.
Pathways
GBA’s enzymatic functions are integral to the glycosphingolipid metabolic pathway. It is involved in the downstream steps of the lysosomal degradation of glycosphingolipids. The pathway operates alongside other important proteins such as beta-glucosidase and CERT-related transfer proteins all of which contribute to membrane lipid organization and signal transduction processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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