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GBP2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.

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Images

Western blot - Human GBP2 knockout A549 cell lysate (AB257962), expandable thumbnail
  • Sanger Sequencing - Human GBP2 knockout A549 cell lysate (AB257962), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.

Alternative names

GTP-binding protein 2, Guanine nucleotide binding protein 2, Guanylate binding protein 2 interferon inducible, HuGBP 2, Interferon induced guanylate binding protein 2

What's included?

1 Kit
Components
Human GBP2 knockout A549 cell lysate
1 x 100 µg
Human wild-type A549 cell lysate
1 x 100 µg

Recommended products

GBP2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.

Key facts

Cell type
A549
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6.
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
GBP2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GBP2 also known as Guanylate Binding Protein 2 is a member of the dynamin superfamily of large GTPases. It has an approximate mass of 67 kDa. This protein finds expression predominantly in macrophages and other immune cells. The involvement of GBP2 in cellular defense mechanisms reflects its upregulation by interferon-gamma where it takes part in immune responses against microbial and viral infections.

Biological function summary

Guanylate Binding Protein 2 participates in immune response regulation. It does not function as part of a large multiprotein complex but acts primarily as a monomer. GBP2 disrupts pathogenic molecular machinery by binding to microbial enzymes leading to their inhibition which limits pathogen survival. This mechanism ensures that GBP2 remains a significant factor in the cell's intrinsic ability to fight infections at the molecular level.

Pathways

Guanylate Binding Protein 2 plays an important role in the interferon-induced defense pathways. It participates in the immune effector pathways primarily connecting to processes like the JAK-STAT signaling pathway. Through this involvement GBP2 helps enhance the expression of a variety of immunologically relevant genes. Additionally GBP2 often interacts with other members of the GBP family including GBP1 and GBP5 to amplify immune responses and signaling cascades.

Associated diseases and disorders

Guanylate Binding Protein 2 shows a significant connection to infectious diseases like tuberculosis and viral infections such as hepatitis C. Overexpression or dysregulation of GBP2 can influence the progression and severity of these conditions. Additionally GBP2's interaction with proteins involved in inflammatory responses such as other interferon-induced proteins highlights its influence over auto-inflammatory conditions and its potential as a biomarker for disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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