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AB263214

Human GDE1 (MIR16) knockout HeLa cell lysate

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GDE1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

View Alternative Names

363E6.2, EC 3.1.4.44, Glycerophosphodiester phosphodiesterase 1, Membrane interacting protein of RGS16, RGS16 interacting membrane protein

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Sanger Sequencing - Human GDE1 (MIR16) knockout HeLa cell lysate (AB263214)
  • Sanger seq

Unknown

Sanger Sequencing - Human GDE1 (MIR16) knockout HeLa cell lysate (AB263214)

Homozygous : 1 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
GDE1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIR16 also known as microRNA-16 is a small non-coding RNA molecule typically around 22 nucleotides in length. This microRNA engages in the post-transcriptional regulation of gene expression impacting various mRNAs by binding and either degrading them or inhibiting their translation. MIR16 is expressed in numerous human tissues including the lungs heart and spleen indicating its broad functional significance in the body.
Biological function summary

MIR16 regulates cell cycle progression and apoptosis playing a role in maintaining cellular homeostasis. It is part of the microRNA-induced silencing complex (RISC) enabling it to mediate gene silencing efficiently. By influencing the expression of genes involved in the cell cycle MIR16 helps control cell proliferation and programmed cell death which are important processes for normal cellular function and response to stress.

Pathways

MIR16 is involved in the cell cycle and apoptosis signaling pathways. It interacts with key proteins such as cyclin D1 and Bcl-2 which are essential for cell cycle regulation and apoptosis respectively. By modulating these pathways MIR16 contributes to maintaining the balance between cell survival and death which is necessary to prevent abnormal cell growth.

MIR16 has connections to cancer and cardiovascular diseases. Aberrant expression of MIR16 can lead to uncontrolled cell proliferation linking it to cancer progression particularly in chronic lymphocytic leukemia where it shows reduced expression. Moreover its role in regulating apoptosis implicates MIR16 in cardiovascular diseases where its dysregulation can impact heart function. The interaction with proteins such as Bcl-2 in these conditions highlights its potential as a therapeutic target in disease management.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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