Human GHITM (MICS1) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
GHITM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
View Alternative Names
DERP2, Dermal papilla-derived protein 2, GHITM_HUMAN, Growth hormone-inducible transmembrane protein, HSPC282, MICS1, Mitochondrial morphology and cristae structure 1, My021, PTD010, TMBIM5, Transmembrane BAX inhibitor motif containing 5, Transmembrane BAX inhibitor motif-containing protein 5
- Sanger seq
Unknown
Sanger Sequencing - Human GHITM (MICS1) knockout HEK-293T cell lysate (AB258881)
Homozygous : Insertion of the selection cassette in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MICS1 contributes to the establishment of the mitochondrial inner membrane potential essential for maintaining proper mitochondrial function. MICS1 forms part of the translocase of inner membrane (TIM) complex which facilitates the import of preproteins into the mitochondria. This activity supports cellular energy metabolism by ensuring efficient electron transport chain operation and ATP production.
Pathways
MICS1 is involved in the mitochondrial protein import pathway and the maintenance of mitochondrial membrane potential. It interacts with other components of the TIM complex such as Tim23 and works closely with proteins in the oxidative phosphorylation pathway. These interactions ensure a seamless integration of imported mitochondrial proteins into the inner membrane facilitating cellular energy production processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com