Human GLYR1 knockout HEK-293T cell lysate
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GLYR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp insertion in exon 3.
View Alternative Names
3-hydroxyisobutyrate dehydrogenase-like protein, BM045, Cytokine-like nuclear factor N-PAC, GLYR1_HUMAN, Glyoxylate reductase 1 homolog, HIBDL, N-PAC, NP60, Nuclear protein 60 kDa, Nuclear protein NP60, Nuclear protein of 60 kDa, Putative oxidoreductase GLYR1
- WB
Unknown
Western blot - Human GLYR1 knockout HEK-293T cell lysate (AB257968)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : GLYR1 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : HUVEC cell lysate (20 ug)
ab167155 was shown to specifically react with GLYR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266610 (knockout cell lysate ab257968) was used. Wild-type and GLYR1 knockout samples were subjected to SDS-PAGE. ab167155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- WB
Unknown
Western blot - Human GLYR1 knockout HEK-293T cell lysate (AB257968)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : GLYR1 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : HUVEC cell lysate (20 ug)
ab154838 was shown to specifically react with GLYR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266610 (knockout cell lysate ab257968) was used. Wild-type and GLYR1 knockout samples were subjected to SDS-PAGE. ab154838 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human GLYR1 knockout HEK-293T cell lysate (AB257968)
Homozygous : 10 bp insertion in exon 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GLYR1 influences chromatin structure by regulating histone H3 lysine 36 trimethylation (H3K36me3). It interacts with lysine-specific demethylase 1 (LSD1) and partners within chromatin-modifying complexes. Through these interactions GLYR1 can impact transcriptional repression and activation. Its activity shows specific importance in controlling gene expression related to cell growth and differentiation.
Pathways
GLYR1 interacts with components of the MAPK signaling pathway and the histone modification pathway. It works alongside proteins like LSD1 and histone deacetylases (HDACs) to modulate transcriptional outcomes. These interactions provide evidence of its role in transmitting extracellular signals to the nucleus ultimately influencing gene expression patterns.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-GLYR1 antibody [EPR10077(B)] (AB167155)](https://content.abcam.com/products/images/glyr1-antibody-epr10077b-ab167155--western-blot-img438.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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