Human GMCSF knockout HEK-293T cell lysate
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CSF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1.
View Alternative Names
CSF, CSF2_HUMAN, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, GM-CSF, Granulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, MGI1GM, Molgramostin, Pluripoietin-a, Sargramostim
- Sanger seq
Unknown
Sanger Sequencing - Human GMCSF knockout HEK-293T cell lysate (AB263219)
Homozygous : 11 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.
Pathways
GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB252634
Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector)
RabMAb|Recombinant
![Sandwich ELISA - Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector) (AB252634)](https://content.abcam.com/products/images/gm-csf-antibody-epr22272-71-bsa-and-azide-free-detector-ab252634--sandwich-elisa-img52778.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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