GMPR KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 74 bp deletion in exon4.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 74 bp deletion in exon4.
Alternative names
GMP reductase 1, GMPR1_HUMAN, Gmpr, Guanosine 5' monophosphate oxidoreductase 1, Guanosine 5''-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1, guanosine monophosphate reductase
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GMPR KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 74 bp deletion in exon4.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 74 bp deletion in exon4.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- GMPR
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GMPR1 protein also known as guanosine monophosphate reductase 1 acts mechanistically as an enzyme that catalyzes the conversion of guanosine monophosphate (GMP) to inosine monophosphate (IMP). This reaction is part of the purine nucleotide cycle important for maintaining the balance of nucleotide pools in cells. GMPR1 has a molecular mass of approximately 37 kDa. Expression of GMPR1 is detected in various tissues with significant levels present in liver and kidney tissues.
- Biological function summary
GMPR1 plays a critical role in purine metabolism by regulating the intracellular concentrations of guanine nucleotides. It does not work as part of a larger complex but functions independently to achieve its effects on nucleotide turnover. This protein ensures efficient recycling of guanine nucleotides which is important for cellular processes such as DNA and RNA synthesis signalling and energy metabolism.
- Pathways
GMPR1 is involved mainly in the purine salvage pathway and the nucleotide degradation pathway. Through these pathways GMPR1 links with other proteins like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which also participates in nucleotide salvage. The balance achieved in these pathways is essential for cellular proliferation and energy conservation highlighting GMPR1's role in overall cellular homeostasis.
- Associated diseases and disorders
GMPR1 has connections to conditions like gout and certain types of cancer where there is an imbalance in purine metabolism. The misregulation of GMPR1 potentially affecting the pathways it participates in may lead to excess uric acid production seen in gout or altered nucleotide metabolism in tumorigenesis. Additionally proteins such as HGPRT which interacts within the same purine pathways also exhibit associations with these disorders further illustrating the significant impact of purine metabolism in human health.
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1 product image
Sanger Sequencing - Human GMPR (GMPR1) knockout A549 cell lysate (ab258435)
Homozygous: 74 bp deletion in exon4
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