Human GNAI1 knockout HEK-293T cell lysate
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GNAI1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp insertion in exon3.
View Alternative Names
Adenylate cyclase inhibitory protein, Adenylate cyclase-inhibiting G alpha protein, G protein alpha inhibiting 1, G protein alpha inhibiting activity polypeptide 1, GNAI1_HUMAN, Gi inhibitory G protein, Gi1 protein alpha subunit, Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1, Guanine nucleotide binding protein G(i) alpha 1 subunit, Guanine nucleotide binding protein alpha inhibiting activity polypeptide 1, Guanine nucleotide-binding protein G(i) subunit alpha-1, OTTHUMP00000161319, OTTHUMP00000208047
- Sanger seq
Unknown
Sanger Sequencing - Human GNAI1 knockout HEK-293T cell lysate (AB257970)
Homozygous : 13 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GNAI1 acts by interfering with signal transmission across membranes. It is part of the heterotrimeric G-protein complex which is essential for many signaling processes. This complex responds to activation by G-protein-coupled receptors (GPCRs) and modulates various downstream effectors essentially affecting numerous physiological processes like neurotransmission and hormone signaling.
Pathways
GNAI1 plays a significant role in the cyclic AMP (cAMP) signaling pathway and the neuroactive ligand-receptor interaction pathway. In the cAMP pathway GNAI1 associates with receptors like dopamine and serotonin receptors helping to regulate neuronal response and neurotransmitter release. This activity involves interactions with related proteins such as GNB1 and GNG2 which form the beta and gamma subunits of the heterotrimeric G-protein complex.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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