Human GNB2 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
GNB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
G protein beta 2 subunit, G protein subunit beta-2, GBB2_HUMAN, Gnb2l1, Guanine nucleotide binding protein G I G S G T beta 2 subunit 2, Guanine nucleotide binding protein G protein beta polypeptide 2, Guanine nucleotide binding protein beta 2 subunit, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, OTTHUMP00000174601, OTTHUMP00000174602, Receptor for activated C kinase, Receptor of activated protein kinase C 1, Signal transducing guanine nucleotide binding regulatory protein beta, Transducin beta chain 2
- WB
Lab
Western blot - Human GNB2 knockout HEK-293T cell lysate (AB257453)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : GNB2 knockout HeLa cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : SH-SY5Y cell lysate (20 ug)
ab108504 was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GNB2 antibody [EP3262Y] (<a href='/en-us/products/primary-antibodies/gnb2-antibody-ep3262y-ab108504'>ab108504</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GNB2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GNB2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gnb2-knockout-hek-293t-cell-line-ab266528'>ab266528</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
SH-SY5Y cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GNB2 knockout HEK-293T cell lysate (AB257453)
Homozygous : 1 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
In the context of cellular processes GNB2 is involved in transmitting signals from activated G protein-coupled receptors to different intracellular effectors. It forms an essential part of the G protein complex which plays a role in facilitating the exchange of GDP for GTP on the alpha subunit. The activation of the G protein complex enables downstream signaling that governs multiple physiological responses such as sensory perception and cellular communication.
Pathways
GNB2 is heavily involved in pathways related to signal transduction and sensory perception. It plays a significant role within the GPCR (G protein-coupled receptor) signaling pathway an important pathway involved in transmitting signals from diverse stimuli outside a cell to its interior. In this context GNB2 interacts with related proteins such as GNAI2 contributing to the regulation of ion channels adenylyl cyclases and other effectors. This connectivity enables precise cellular responses to external signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-GNB2 antibody [EP3262Y] (AB108504)](https://content.abcam.com/products/images/gnb2-antibody-ep3262y-ab108504--western-blot-img421993.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com