Human GNB4 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
GNB4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 7 bp deletion in exon 2.
View Alternative Names
G protein beta 4 subunit, GBB4_HUMAN, GNB 4, Guanine nucleotide binding protein (G protein) beta polypeptide 4, Guanine nucleotide binding protein beta 4 subunit, Guanine nucleotide binding protein beta polypeptide 4, Guanine nucleotide binding protein beta subunit 4, Guanine nucleotide-binding protein subunit beta-4, Transducin beta chain 4
- Sanger seq
Unknown
Sanger Sequencing - Human GNB4 knockout HeLa cell lysate (AB258884)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human GNB4 knockout HeLa cell lysate (AB258884)
Allele-1 : 7 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functionality of GNB4 involves interactions within the heterotrimeric G protein complex a critical component of signal transduction pathways. This protein acts as a modulator or adapter in transmembrane signaling systems. GNB4 pairs with gamma subunits to help regulate downstream effector proteins expanding the impact of receptor-binding events initiated by external stimuli. GNB4 influences processes like cell communication and neurotransmission making it an important factor in maintaining cellular functions in neural networks.
Pathways
GNB4 plays essential roles in G-protein coupled receptor (GPCR) signaling and other signaling pathways. In GPCR signaling GNB4 works in concert with alpha and gamma subunits to mediate signal propagation. The complex modulates cyclic AMP levels and activates phospholipase C affecting various cellular responses. GNB4 interacts closely with proteins such as GNAS and ADCY1 forming part of the intricate signaling networks that drive cellular communication and response mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com