Human GNG5 knockout HEK-293T cell lysate
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GNG5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2 and 7 bp deletion in exon 2.
View Alternative Names
FLJ92393, GBG5_HUMAN, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5, OTTHUMP00000011474, OTTHUMP00000011565
- Sanger seq
Unknown
Sanger Sequencing - Human GNG5 knockout HEK-293T cell lysate (AB258885)
Allele-2 : 2 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human GNG5 knockout HEK-293T cell lysate (AB258885)
Allele-3 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human GNG5 knockout HEK-293T cell lysate (AB258885)
Allele-1 : 7 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GNG5 functions as part of the larger G protein complex. It interacts with G alpha and G beta subunits to form the complete heterotrimeric G protein essential for GPCR signal transduction. By facilitating GDP-GTP exchange in the G alpha component GNG5 helps in activating downstream signaling cascades that influence physiological functions. Its involvement in cellular communication highlights its importance in biological processes like sensory perception and hormone responses.
Pathways
GNG5 participates importantly in the G protein signaling pathway and the GPCR downstream pathway. During signal transduction GNG5 interacts with the G beta subunit to maintain the functional G protein state. This protein also has a relationship with other G proteins such as GNAI1 (Guanine Nucleotide Binding Protein Alpha Inhibiting Activity Polypeptide 1) reflecting its coordination in complex signaling pathways. These pathways are integral to key cellular responses and adaptability to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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