GOLGA5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.
Alternative names
Cell proliferation inducing gene 31, Cell proliferation-inducing gene 31 protein, GOGA5_HUMAN, GOLGA 5, GOLIM 5, Golgi autoantigen golgin subfamily a 5, Golgi integral membrane protein 5, Golgin A5, Golgin subfamily A member 5, Golgin-84, PTC5, Protein Ret-II, RET-fused gene 5 protein, RFG 5, Ret II, Ret II protein, Ret fused gene 5
What's included?
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GOLGA5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.
- Concentration
Properties
- Gene name
- GOLGA5
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GOLGA5 also known as Golgin-84 is an important player in maintaining the structural integrity of the Golgi apparatus. This protein weighs approximately 84 kDa and exists within the Golgi membrane structure. It facilitates the tethering and fusion of vesicles ensuring the proper flow of proteins and lipids through the Golgi network. Golgin-84 expresses in a variety of tissues with strong local presence in cells undergoing secretion indicating its role in cellular transport processes.
- Biological function summary
GOLGA5 participates in the assembly of the Golgi ribbon a structure essential for the efficient functioning of the Golgi apparatus. This protein integrates into a multiprotein complex which is critical for maintaining the stacked and ribbon-like structure of the Golgi. Its interaction with other Golgi matrix proteins helps regulate the positioning and coherence of Golgi cisternae. This ensures proper vesicle transfer and contributes to overall cellular homeostasis.
- Pathways
The function of GOLGA5 is critical in the secretory pathway where it ensures the orderly transport of proteins processed by the Golgi apparatus. The presence of GOLGA5 supports Golgi reassembly and stacking protein 65 (GRASP65) involvement in Golgi reformation during the cell cycle. Its function also connects with ER-to-Golgi transport helping maintain an important support system for intracellular trafficking.
- Associated diseases and disorders
GOLGA5 appears related to neurodegenerative diseases and cancer. Alterations in its function or expression could dispose cells to develop cellular transport disruptions potentially leading to illnesses like Alzheimer’s disease where protein transport and processing are compromised. Furthermore in cases of cancer such as prostate cancer changes in GOLGA5 expression might disrupt normal cell function linking it to pathways involving oncoproteins.
Product promise
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2 product images
Sanger Sequencing - Human GOLGA5 (Golgin-84) knockout HEK-293T cell lysate (ab258887)
Allele-2: 1 bp insertion in exon5
Sanger Sequencing - Human GOLGA5 (Golgin-84) knockout HEK-293T cell lysate (ab258887)
Allele-1: 2 bp deletion in exon5
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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