Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate
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GOLIM4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1.
View Alternative Names
GOLPH4, GIMPC, GIMPc, Golgi integral membrane protein 4, GPP130, Golgi phosphoprotein of 130 kDa, GOLIM4, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa
- WB
Lab
Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate (AB257455)
Lane 1 : Wild-type HEK293T cell lysate (20 μg)
Lane 2 : GOLIM4 knockout HEK293T cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MOLT-4 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab197595 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.
ab197595 Anti-GOLPH4/GPP130 antibody [EPR13439] was shown to specifically react with GOLPH4/GPP130 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266123 (knockout cell lysate ab257455) was used. Wild-type and GOLPH4/GPP130 knockout samples were subjected to SDS-PAGE. ab197595 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (<a href='/en-us/products/primary-antibodies/golph4-gpp130-antibody-epr13439-ab197595'>ab197595</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
GOLIM4 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-golim4-golph4-gpp130-knockout-hek-293t-cell-line-ab266123'>ab266123</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
MOLT-4 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 82 kDa
Observed band size: 130 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GOLIM4 (GOLPH4/GPP130) knockout HEK-293T cell lysate (AB257455)
Homozygous : 2 bp insertion in exon 1
Complementary Products
Primary Antibodies
AB197595
Anti-GOLPH4/GPP130 antibody [EPR13439]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (AB197595)](https://content.abcam.com/products/images/golph4-gpp130-antibody-epr13439-ab197595--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65147.jpg)
- 3
- 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of GOLPH4 centers around its involvement in the protein trafficking process within cells. It acts as part of the complex tasked with maintaining Golgi organization which is important for facilitating accurate cargo sorting and delivery. GOLPH4's location and structure enable it to interact with motor proteins and other Golgi matrix proteins aiding in vesicle tethering and fusion processes.
Pathways
GOLPH4 is involved in essential cellular pathways such as the secretion and endocytic trafficking pathways. It interacts with other Golgi-resident proteins like GM130 and giantin which are essential for the movement of materials through the Golgi and to their final destinations. These pathways ensure that proteins and lipids reach their intended areas within the cell or are secreted to the extracellular space which is important for maintaining cellular function and homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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