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AB257976

Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate

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GOLM1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

View Alternative Names

Golgi phosphoprotein 2, Golgi membrane protein 1, C9orf155, PSEC0242, Golgi membrane protein GP73, UNQ686/PRO1326, GOLPH2, GOLM1

3 Images
Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)
  • WB

Lab

Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)

Lane 1 : Wild-type HEK-293T cell lysate, 10 ug

Lane 2 : GOLM1 knockout HEK-293T cell lysate, 10 ug

Lane 3 : U-2 OS cell lysate, 20 ug

Lane 4 : HepG2 cell lysate, 20 ug

Lane 5 : MDA-MB-231 cell lysate, 20 ug

Lane 6 : LNCaP cell lysate, 20 ug

False colour image of Western blot : Anti-GOLPH2 antibody [OTI6C9] staining at 1/500 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab119800 was shown to bind specifically to GOLPH2. A band was observed at 70-75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GOLM1 knockout cell line ab266505 (knockout cell lysate ab257976). To generate this image, wild-type and GOLM1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

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Sanger Sequencing - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)
  • Sanger seq

Unknown

Sanger Sequencing - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)

Homozygous : 1 bp insertion in exon 3

Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)
  • WB

Supplier Data

Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (AB257976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : HepG2. In Western blot, ab320737 was shown to bind specifically to GOLM1 (GOLPH2). Target of interest was observed at 75 kDa in wild-type HEK-293T cell lysates (lane 1) with no signal observed at this size in the GOLM1 (GOLPH2) knockout cell line (lane 2) (knockout cell line ab266505 / knockout cell lysate ab257976). The identity of the band above 75KDa in lanes 5 and 7 is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (<a href='/en-us/products/primary-antibodies/golm1-golph2-antibody-epr29022-61-ab320737'>ab320737</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell lysate (ab257976) at 20 µg

Lane 3:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

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Exposure time: 70s

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

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Complementary Products

Primary Antibodies

AB92612

Anti-GOLPH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (AB92612)

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Cell Lines & Lysates

AB266505

Human GOLM1 (GOLPH2) knockout HEK-293T cell line

Advanced Validation

Western blot - Human GOLM1 (GOLPH2) knockout HEK-293T cell line (AB266505)

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Proteins & Peptides

AB276495

Recombinant Human GOLPH2 protein (His tag)

SDS-PAGE - Recombinant Human GOLPH2 protein (His tag) (AB276495)

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Assay Kits

AB277428

Human GOLPH2 ELISA Kit

ELISA - Human GOLPH2 ELISA Kit (AB277428)

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  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
GOLM1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GOLPH2 also known as GOLM1 or Golgi membrane protein 1 has a significant mechanical role in the cell. It is a 73-kilodalton protein found mainly in the Golgi apparatus. This protein gets expressed in various tissues with notable levels in the liver and other organs involved in secretion. It acts as a transmembrane protein in the Golgi where it helps in processing and sorting secretory proteins influencing how proteins move through secretory pathways.
Biological function summary

GOLPH2 plays an essential role in maintaining Golgi function and efficient protein trafficking. It does not belong to a large protein complex but its proper functioning is critical for handling secretory pathways. The protein keeps secretory flow organized which affects cellular responses and interactions with the environment. The GOLPH2 protein also has connections to immunity responding to stimuli like infections or inflammation.

Pathways

GOLPH2 actively participates in secretory pathways and protein transport systems. Its role includes coordination with other Golgi proteins to facilitate protein modification and sorting. GOLPH2 interacts with other proteins like GOLM1 and GOLPH3 impacting the Golgi's structural organization and influencing pathways like vesicular transport. These interactions are important for adapting the intracellular environment to external demands.

GOLPH2 levels change in diseases such as liver cancer and hepatitis. These changes can serve as biomarkers for diagnosis and disease progression monitoring. GOLPH2 also connects with proteins involved in these diseases including AFP a known tumor marker. Understanding GOLPH2's role in such conditions aids research in developing diagnostic tools and therapeutic strategies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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