Human GPC4 (Glypican 4) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human GPC4 (Glypican 4) knockout HEK-293T cell lysate (AB257457)
Allele-2 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human GPC4 (Glypican 4) knockout HEK-293T cell lysate (AB257457)
Allele-1 : 14 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Glypican 4 influences cell growth differentiation and morphogenesis. It interacts with extracellular ligands and growth factors playing a role in developmental processes. Glypican 4 is often part of a complex with other signaling molecules including Wnt and fibroblast growth factors. These interactions help regulate processes essential for organ development and tissue patterning. Glypican 4 through these molecular associations influences signaling pathways that affect both cellular architecture and function.
Pathways
Glypican 4 plays a role in Wnt signaling and Hedgehog pathways. These pathways are critical for embryonic development and cell fate determination. Within the Wnt signaling pathway Glypican 4 interacts with the Wnt protein modulating its distribution and activity on the cell surface. In the Hedgehog pathway Glypican 4 helps modulate hedgehog proteins like Sonic Hedgehog facilitating their movement and interaction with cell surface receptors.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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