GPC4 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 1 bp insertion in exon3.
Images
Key facts
- Cell type
HEK-293T
- Species or organism
Human
- Tissue
Kidney
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 1 bp insertion in exon3.
Alternative names
GPC4_HUMAN, Glypican proteoglycan 4, K-glypican, OTTHUMP00000024052, Secreted glypican-4, UNQ474/PRO937, dJ900E8.1 glypican 4
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GPC4 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 1 bp insertion in exon3.
Key facts
- Cell type
HEK-293T
- Species or organism
Human
- Tissue
Kidney
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 1 bp insertion in exon3.
- Concentration
Properties
- Gene name
GPC4
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glypican 4 also known as GPC4 is a heparan sulfate proteoglycan a member of the glypican family. This protein is anchored to the cell membrane and plays a role in cellular processes by attaching to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. Its mass is approximately 60-70 kDa. Glypican 4 is expressed in various tissues including the brain pancreas kidney and skeletal muscle. In these tissues Glypican 4 is involved in cell communication and modulation of signaling activities at the cell surface.
- Biological function summary
Glypican 4 influences cell growth differentiation and morphogenesis. It interacts with extracellular ligands and growth factors playing a role in developmental processes. Glypican 4 is often part of a complex with other signaling molecules including Wnt and fibroblast growth factors. These interactions help regulate processes essential for organ development and tissue patterning. Glypican 4 through these molecular associations influences signaling pathways that affect both cellular architecture and function.
- Pathways
Glypican 4 plays a role in Wnt signaling and Hedgehog pathways. These pathways are critical for embryonic development and cell fate determination. Within the Wnt signaling pathway Glypican 4 interacts with the Wnt protein modulating its distribution and activity on the cell surface. In the Hedgehog pathway Glypican 4 helps modulate hedgehog proteins like Sonic Hedgehog facilitating their movement and interaction with cell surface receptors.
- Associated diseases and disorders
Glypican 4 is connected to some cancers and metabolic disorders like diabetes. Overexpression or mutations in Glypican 4 can contribute to oncogenesis as it may lead to aberrant activation of signaling pathways making it relevant in cancers like pancreatic and brain tumors. Additionally Glypican 4's role in metabolic pathways links it to insulin-related pathways emphasizing its connection with metabolic disorders such as Type 2 diabetes. Glypican 4 interacts with proteins such as insulin receptors which further highlights its relevance in these conditions.
Product promise
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2 product images
Sanger Sequencing - Human GPC4 (Glypican 4) knockout HEK-293T cell lysate (ab257457)
Allele-1: 14 bp deletion in exon3
Sanger Sequencing - Human GPC4 (Glypican 4) knockout HEK-293T cell lysate (ab257457)
Allele-2: 1 bp insertion in exon3
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- Visit the general protocols
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