AB256932

Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate

Name: Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (ab256932) | Abcam
Brand: Abcam Limited
SKU: AB256932

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GPX1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

3 Images

Lab

Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : GPX1 knockout HEK-293T cell lysate 20 μg
False colour image of Western blot : Anti-Glutathione Peroxidase 1 antibody [EPR3311] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108429 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line ab266650 (knockout cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] (<a href='/en-us/products/primary-antibodies/glutathione-peroxidase-1-antibody-epr3311-ab108429'>ab108429</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

GPX1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gpx1-glutathione-peroxidase-1-knockout-hek-293t-cell-line-ab266650'>ab266650</a>)

Predicted band size: 22 kDa

Observed band size: 22 kDa

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Lab

Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : GPX1 knockout HEK-293T cell lysate 20 μg
False colour image of Western blot : Anti-Glutathione Peroxidase 1 antibody [EPR3312] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108427 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line ab266650 (knockout cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3312] (<a href='/en-us/products/primary-antibodies/glutathione-peroxidase-1-antibody-epr3312-ab108427'>ab108427</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

GPX1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Predicted band size: 22 kDa

Observed band size: 22 kDa

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Sanger Sequencing - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Sanger seq

Unknown

Sanger Sequencing - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256932-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human GPX1 knockout HEK293T cell lysate", "number":"AB256932-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB256932-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Gene name

GPX1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Zygosity

Homozygous

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 1 (GPx1) also known as GSH peroxidase is a selenium-containing enzyme with a mass of approximately 22 kDa. It plays an important role in reducing hydrogen peroxide to water using glutathione as a substrate therefore protecting cells from oxidative damage. GPx1 is widely expressed in many tissues but is found in high concentrations in the liver and erythrocytes. Researchers use antibodies specific to GPx1 often obtained from specialized suppliers to study and quantify this enzyme in various biological samples.

Biological function summary

The enzyme guards cellular components by neutralizing free radicals. GPx1 is not part of a large complex but functions as a homotetramer. Its activity is essential for preserving the redox balance within the cell preventing cellular damage from reactive oxygen species (ROS). Enzyme-linked immunosorbent assays (ELISA) often measure GPx1 activity to assess oxidative stress levels in research and clinical settings.

Pathways

GPx1 is important in the glutathione metabolism and cellular antioxidant defense pathways. Within these pathways GPx1 works closely with proteins like glutathione reductase to maintain reduced glutathione levels. This interplay with other antioxidant proteins ensures cellular protection against oxidative damage. Studies focus on GPx1's role in these pathways as it underpins many critical cellular processes maintaining cell health.

Decreased GPx1 activity links to neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases such as Alzheimer's the oxidative stress overwhelms the antioxidant defenses including GPx1. Similarly in cardiovascular disorders a diminished GPx1 activity correlates with increased oxidative stress contributing to disease progression. Researchers also investigate connections between GPx1 and other oxidative stress-related proteins like superoxide dismutase to fully understand GPx1's role in disease mechanisms.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate

Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Western blot - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Sanger Sequencing - Human GPX1 (Glutathione Peroxidase 1) knockout HEK-293T cell lysate (AB256932)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Complementary Products

Primary Antibodies

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Zygosity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Product promise