Human GRHPR knockout HEK-293T cell lysate
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GRHPR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon1.
View Alternative Names
GLXR, GLYD, GRHPR_HUMAN, Glyoxylate reductase/hydroxypyruvate reductase, OTTHUMP00000021379, OTTHUMP00000021380, OTTHUMP00000046131, PH 2, Primary hyperoxaluria type 2, glycerate 2 dehydrogenase
- Sanger seq
Unknown
Sanger Sequencing - Human GRHPR knockout HEK-293T cell lysate (AB258889)
Homozygous : 29 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRHPR acts as a detoxifying agent by reducing glyoxylate levels protecting cells from glyoxylate-mediated toxicity. This enzyme is not part of a larger complex and functions independently. Well-balanced glyoxylate and glycolate levels are critical for cellular homeostasis and GRHPR ensures proper maintenance and regulation within metabolic pathways.
Pathways
GRHPR participates in glyoxylate and dicarboxylate metabolism helping control the flow of metabolites. This involvement is significant in the context of the glyoxylate cycle and serine metabolism. Additionally it interacts with proteins such as lactate dehydrogenase during its activity within these metabolic pathways demonstrating its interconnectedness within cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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