Human GRK4 knockout HeLa cell lysate
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GRK4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and 31 bp deletion in exon9.
View Alternative Names
G protein-coupled receptor kinase 2 (Drosophila)-like, G protein-coupled receptor kinase 4, G protein-coupled receptor kinase GRK4, G proteinm coupled receptor kinase GRK4, GPRK2L, GPRK4, GRK4_HUMAN, GRK4a, ITI1
- Sanger seq
Unknown
Sanger Sequencing - Human GRK4 knockout HeLa cell lysate (AB258441)
Allele-1 : 31 bp deletion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human GRK4 knockout HeLa cell lysate (AB258441)
Allele-2 : 1 bp deletion in exon9
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
G protein-coupled receptor kinase 4 regulates GPCR signaling by facilitating receptor internalization and degradation. This action modulates cellular responsiveness to hormones and neurotransmitters. GRK4 does not form part of a complex but its regulatory function directly impacts GPCR pathways. GRK4 achieves this control by adding phosphate groups typically functioning as a feedback mechanism during receptor activity.
Pathways
GRK4 serves as an important regulator in the MAPK signaling pathway and the renin-angiotensin-aldosterone system (RAAS). In MAPK pathways GRK4 helps mediate signal transduction by controlling receptor activity. It also interacts with other GRKs like GRK2 to fine-tune cellular responses and maintain homeostasis. Its involvement in RAAS highlights its significance in cardiovascular regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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