AB257461

Human GSTK1 knockout HEK-293T cell lysate

Name: Human GSTK1 knockout HEK-293T cell lysate (ab257461) | Abcam
Brand: Abcam Limited
SKU: AB257461

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GSTK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

HDCMD47P, Glutathione S-transferase kappa 1, GSTK1, hGSTK1, Glutathione S-transferase subunit 13, GST class-kappa, GSTK1-1, GST 13-13

3 Images

Lab

Western blot - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : GSTK1 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Daudi cell lysate (20 ug)

ab52759 was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used. Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE. ab52759 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GSTK1 antibody [EP1938Y] (<a href='/en-us/products/primary-antibodies/gstk1-antibody-ep1938y-ab52759'>ab52759</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

GSTK1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human GSTK1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gstk1-knockout-hek-293t-cell-line-ab266112'>ab266112</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

Lab

Western blot - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : GSTK1 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Daudi cell lysate (20 ug)

ab134173 was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used. Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE. ab134173 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GSTK1 antibody [EPR1939] (<a href='/en-us/products/primary-antibodies/gstk1-antibody-epr1939-ab134173'>ab134173</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

GSTK1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human GSTK1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-gstk1-knockout-hek-293t-cell-line-ab266112'>ab266112</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

Sanger Sequencing - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Sanger seq

Unknown

Sanger Sequencing - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

GSTK1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Zygosity

Homozygous

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTK1 also known as Glutathione S-transferase kappa 1 is an enzyme with a mass of approximately 26 kDa. This protein belongs to the glutathione S-transferase family which plays a significant role in detoxification processes through conjugation reactions. GSTK1 displays a broad tissue expression with abundant presence in liver and kidneys as well as other tissues which facilitates its involvement in protecting cells from oxidative stress and chemical damage.

Biological function summary

GSTK1 is part of the cellular defense mechanism against xenobiotic and oxidative stress. This enzyme participates in the neutralization of harmful compounds by facilitating their conjugation with glutathione a powerful antioxidant. GSTK1 functions independently and does not form part of a larger protein complex. Its activity is important in maintaining cellular homeostasis and preventing damage caused by reactive oxygen species.

Pathways

GSTK1 has critical involvement in glutathione metabolism and xenobiotic detoxification pathways. It interacts functionally with proteins like GSTM1 which are also part of the glutathione S-transferase family enhancing the cell’s ability to detoxify harmful substances. GSTK1 helps to modulate the cellular response to oxidative stress through these pathways ensuring protection against toxicity.

GSTK1 has been linked to conditions including cancer and liver diseases. Its detoxification role makes GSTK1 significant in cancer biology where its expression levels may influence tumor resistance to chemotherapy. Additionally GSTK1 has connections with other glutathione S-transferases like GSTP1 which have associations with similar disorders affecting cellular protection mechanisms.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GSTK1 knockout HEK-293T cell lysate

Western blot - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Western blot - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Sanger Sequencing - Human GSTK1 knockout HEK-293T cell lysate (AB257461)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Zygosity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Product promise