Human GSTM4 knockout HeLa cell lysate
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GSTM4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
GST class-mu 4, GST-Mu2, GSTM4-4, GSTM4_HUMAN, GTM4, GTS Mu2, Glutathione S transferase M4, Glutathione S-transferase Mu 4, MGC131945, MGC9247
- Sanger seq
Unknown
Sanger Sequencing - Human GSTM4 knockout HeLa cell lysate (AB258890)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GSTM4 functions as a detoxification enzyme that protects cells from oxidative stress and damage from harmful compounds. It operates as part of a complex system of enzymes collectively known for their role in phase II metabolism which is important for clearing toxins from the body. By contributing to the neutralization of reactive oxygen species GSTM4 helps maintain cell health and prevent damage to cellular components like DNA proteins and lipids which could otherwise lead to cell dysfunction or death.
Pathways
GSTM4's activity integrates into pathways such as glutathione metabolism and the xenobiotic detoxification pathway. These pathways utilize GSTM4 and other proteins including the glutathione itself to neutralize toxic compounds and mitigate cellular stress. Other related proteins in these pathways include various isoforms of Glutathione S-transferases highlighting a cooperative network essential for effective detoxification processes inside the body.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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