Human GSTT2B (GSTT2) knockout HeLa cell lysate
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GSTT2B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
View Alternative Names
AI266894, EC 2.5.1.18, GST 12 12, GST class-theta-2, GSTT2 protein, GSTT2B, GSTT2P, GSTT2_HUMAN, Glutathione S transferase 12, Glutathione S transferase Yrs Yrs, Glutathione S-transferase theta-2, Glutathione S-transferase theta-2B, MGC182032, OTTHUMP00000198491, OTTHUMP00000198492, Yrs, mGSTT2
- WB
Supplier Data
Western blot - Human GSTT2B (GSTT2) knockout HeLa cell lysate (AB258443)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : GSTT2B knockout HeLa cell lysate (20 μg)
Lane 3 : Human lung tissue lysate (20 μg)
Lane 4 : A549 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GSTT2 antibody [EPR8136(2)] (<a href='/en-us/products/primary-antibodies/gstt2-antibody-epr81362-ab176336'>ab176336</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GSTT2B knockout HeLa cell lysate at 20 µg
Lane 3:
Human lung tissue lysate at 20 µg
Lane 4:
A549 cell lysate at 20 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GSTT2B (GSTT2) knockout HeLa cell lysate (AB258443)
Homozygous : 2 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GSTT2 facilitates the detoxification process by catalyzing reactions that neutralize harmful compounds. It catalyzes the conjugation of glutathione to form less reactive electrophilic or hydrophobic substances. GSTT2 does not function as part of a larger complex within cells but has an important role in maintaining the redox balance. The enzyme contributes to cellular defense mechanisms ensuring proper cellular functions in stressful environments.
Pathways
The activity of GSTT2 has connections to the glutathione metabolism and drug metabolism pathways. These pathways are important for neutralizing endogenous and exogenous toxins. In the glutathione metabolism pathway GSTT2 works in conjunction with other GST enzymes like GSTP1 and GSTA1 to manage oxidative stress. The protein indirectly supports cellular detoxification processes influencing how effectively a cell can endure and adapt to toxic insults.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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